石榴果皮提取物纳米氧化铜抑菌纸的制备及性能研究

Preparation and property of nano-copper oxide antibacterial paper of pomegranate pericarp extract

关键词:  石榴  多酚  安石榴苷  纳米氧化铜  抑菌纸

Key words:  pomegranate  polyphenol  punicalagin  nano-copper oxide  antibacterial paper

摘要:

目的　制备石榴果皮提取物的纳米氧化铜(CuO) 抑菌纸并分析其性能。方法　采用索氏提取法提取突尼斯 软籽石榴皮中的多酚类物质，利用石榴果皮中的主要多酚类物质安石榴苷与Cu2+ 产生共沉淀，使纳米CuO固定化；采用 水热法制备具有金属+多酚类物质联合抗菌活性的纳米氧化铜抑菌纸；采用抑菌圈测试方法测试所合成抑菌纸的抗菌 性能。结果　成功制备了基于石榴皮甲醇提取液（10 g石榴皮粉末+100 mL甲醇）纳米CuO抑菌纸。其中，抑菌性能最优 制备方案为：所浸渍硫酸铜溶液浓度 1.0 mol/L，浸渍温度 60 ℃，浸渍时间 600 s。此条件下合成的抑菌纸对大肠埃希菌、 金黄色葡萄球菌均有较好的抑制作用，平均抑菌圈直径分别为（20.5±0.8）mm与（18.2±0.4）mm。结论　基于石榴皮甲 醇提取液混合物所合成的纳米CuO抑菌纸对大肠埃希菌及金黄色葡萄球菌都有良好的抑菌效果。

Abstract:

Objective　To prepare nano-copper oxide (CuO) antibacterial paper from pomegranate peel extract and analyze its properties. Methods　The polyphenols in Tunisian soft-seeded pomegranate pericarp were extracted by Soxhlete extraction method, and the main polyphenolic compound, punicalagin, was co-precipitated with Cu2+ to immobilize nano-CuO. A nanoCuO antibacterial paper was prepared with antibacterial activity of metal and polyphenol substances using hydrothermal method. The antibacterial properties of the synthesized antibacterial paper were tested by inhibition circle test. Results　The nano-CuO antibacterial paper was successfully prepared with methanol extract of pomegranate peel (10 g pomegranate peel powder + 100 mL methanol). The optimal preparation scheme of antibacterial performance was: 1.0 mol/L impregnated copper sulfate solution at 60 °C for 600 s. The antibacterial paper synthesized under these conditions showed good inhibitory effects on Escherichia coli and Staphylococcus aureus, with average inhibition zone diameters of (20.5±0.8) mm and (18.2±0.4) mm, respectively. Conclusion　The nano-CuO antibacterial paper synthesized from the mixture of pomegranate peel methanol extract has good antibacterial effects on E. coli and S. aureus.