基于文本挖掘技术探讨针刺治疗肥胖症的作用机制

Exploring the mechanism of acupuncture in the treatment of obesity based on text mining

关键词:  文本挖掘技术  针刺  肥胖症  瘦素  脂联素  胰腺癌  作用机制

Key words:  text mining technology  acupuncture  obesity  leptin  adiponectin  pancreatic adenocarcinoma  mechanism of action

摘要:

目的　通过使用计算工具和公开数据确定肥胖症相关的基因和信号通路，了解针刺治疗肥胖症的潜在机制， 并初步探索针刺在肥胖相关癌症表达中的差异基因。方法　使用文本挖掘的方法，通过GenCLiP 3 检索和筛选出与“肥 胖”“瘦素”“针刺”高度相关的基因，并使用David功能富集进行GO分析和KEGG分析，利用String数据库进行蛋白质 间相互作用分析，最后通过基因表达谱交互分析（GEPIA）数据库对核心基因进行Boxplot和 Survival Analysis分析。结果 筛选出脂联素（ADIPOQ）、瘦素（LEP）、胰岛素（INS）等 14 个关键基因，主要富集于非酒精性脂肪肝、脂肪细胞因子信号 通路、AMPK信号通路、胰岛素分泌以及JAK-STAT信号通路等 10 条通路。GEPIA筛选结果显示白介素 6（IL6）在胰腺 癌（PAAD）的表达中均有重要意义。结论　针刺疗法可能通过调节ADIPOQ、LEP、INS等基因和参与AMPK等信号通 路改善肥胖症，同时针刺可以辅助治疗PAAD，为肥胖症的治疗提供了新的思路。

Abstract:

Objective To identify obesity-related genes and signaling pathways, computational tools and publicly available data were used to analyze the potential mechanisms of acupuncture for the treatment of obesity, and to initially explore the differential genes of acupuncture in the expression of obesity-associated cancers. Methods Text mining methods were used to search the genes highly associated with“obesity”“leptin” and “acupuncture”by GenCLIP 3, and then David’s functional enrichment for GO analysis and KEGG analysis, and the String database was used to identify the difference genes. Finally, the Boxplot and Survival Analysis of the core genes were performed by the Gene Expression Profiling Interaction Analysis (GEPIA) database. Results 14 key genes, including adiponectin (ADIPOQ), leptin (LEP), and insulin (INS), were screened, which were mainly enriched in 10 pathways, including non-alcoholic fatty liver disease, adipocytokine signaling pathway, AMPK signaling pathway, insulin secretion, and JAK-STAT signaling pathway, etc. The results of GEPIA screening showed that interleukin 6 (IL6) was important in the expression of both pancreatic adenocarcinoma (PAAD). Conclusion Acupuncture therapy may improve obesity by regulating genes such as ADIPOQ, LEP, and INS and participating in signaling pathways such as AMPK, while acupuncture can assist in the treatment of PAAD, which provides new ideas for the treatment of obesity.