基于网络药理学和体外实验探讨参芪扶正液对高糖诱导心肌损伤的保护作用

Protective effect of Shenqi Fuzheng liquid on high glucose-induced myocardial injury using network pharmacology and in vitro experiment

关键词:  参芪扶正液  心肌损伤  网络药理学  PI3K/AKT信号通路

Key words:  Shenqi Fuzheng liquid  myocardial injury  network pharmacology  PI3K/AKT signaling

摘要:

目的　探讨参芪扶正液对高糖诱导心肌损伤的潜在作用机制。方法　从TCMSP、GeneCards和OMIM数据 库中分别筛选参芪扶正液活性成分、作用靶点及心肌损伤基因，通过String数据库构建蛋白质相互作用（PPI）网络，使用 Metascape数据库对交集基因进行GO、KEGG分析。H9c2 心肌细胞分别用 35 mmol/L葡萄糖或 1、5 g/L参芪扶正液处 理，MTT法检测细胞存活率，荧光染色检测细胞凋亡，Western blot检测PI3K/AKT表达。结果　发现参芪扶正液 495 个 作用靶点，心肌损伤靶点基因 2 148 个，两者交集基因为 227 个，主要涉及内分泌抵抗、HIF-1、AMPK、PI3K/AKT等信号 通路。参芪扶正液可增加心肌细胞存活，减少细胞凋亡，上调p-PI3K和p-AKT表达（P<0.05）。结论　参芪扶正液可能通 过PI3K/AKT信号减轻高糖诱导的心肌损伤。

Abstract:

Objective To explore the potential mechanism of Shenqi Fuzheng liquid (SFL) on high glucose-induced myocardial injury (MI). Methods The active ingredients and targets of SFL, and MI genes were screened from TCMSP, GeneCards and OMIM databases, respectively. A PPI network was constructed using String database, and the intersection genes were analyzed by GO and KEGG using Metascape database. H9c2 myocardial cells were treated with 35 mmol/L glucose or adding with 1 and 5 g/L SFL. Survival rate, apoptosis and PI3K/AKT expression were detected by MTT, fluorescence staining and Western blot, respectively. Results There were 495 active ingredients of SFL, 2 148 genes of MI, and 227 intersecting genes between them. These intersecting genes were mainly involved in endocrine resistance, HIF-1, AMPK, PI3K/AKT signaling pathways. SFL increased survival rate, reduced apoptosis, and upregulated p-PI3K/p-AKT expression of injured myocardial cells. Conclusion SFL can alleviate high glucose-induced MI through PI3K/AKT signaling.