高效液相色谱法同时测定化妆品中的多种防腐剂

Simultaneous determination of several preservatives in cosmetics using high performance liquid chromatography

关键词:  高效液相色谱法  化妆品  防腐剂

Key words:  HPLC  cosmetics  preservatives

摘要:

目的　建立高效液相色谱法同时测定化妆品中氢醌、苯酚、儿茶酚、苯甲酸及山梨酸等 5 种防腐剂含量方法。 方法　美白类化妆品样品经甲醇溶剂超声辅助提取，C18 色谱柱分离，以 5 mmol/L乙酸铵-1‰甲酸/甲醇溶液（30/70， v/v）为流动相等度洗脱，流速为 1 mL/min，柱温 35 ℃，进样量为 10 μL。结果　氢醌、苯酚、儿茶酚、苯甲酸及山梨酸等 5 种防腐剂在各自线性范围内线性关系良好（r＞0.992），平均加样回收率为 97.82%~111.11%，相对标准偏差为 0.23 %~ 1.64 %。采用该方法对采购的美白类化妆品样品 24 批次进行检测，该法能有效检出化妆品中苯酚、儿茶酚、苯甲酸和山 梨酸，氢醌均未检出。结论　该方法操作简单，灵敏度高，重现性好，可用于美白化妆品中氢醌等 5 种防腐剂的同时检测。

Abstract:

Objective To establish a high-performance liquid chromatography (HPLC) assay for simultaneous detection of 5 preservatives including hydroquinone, phenol, catechol, benzoic acid, and sorbic acid in cosmetics. Methods The whitening cosmetics were extracted ultrasonically with methanol solvent, separated on C18 chromatographic column, and eluted with 5 mmol/L ammonium acetate-1‰ formic acid/methanol solution (30/70) at the flow rate of 1 mL/min. The column temperature was 35 ℃, and injection volume was 10 μL. Results There were good linear relationships among hydroquinone, phenol, catechol, benzoic acid, and sorbic acid (r>0.992). The average recovery rates were 97.82%-111.11%, and relative standard deviations were 0.23%-1.64%. Twenty-four batches of whitening cosmetics were detected by this method. Phenol, catechol, benzoic acid, and sorbic acid were effectively determined, but hydroquinone was not. Conclusion This method is simple, sensitive, and reproducible, and can be used for simultaneous detection of 5 preservatives such as hydroquinone in whitening cosmetics.