甘草素对肺动脉高压大鼠肺血管重塑及右心重构的影响

Glycyrrhizin ameliorates right ventricular remodeling in pulmonary hypertension

关键词:  甘草素  肺动脉高压  右心重构  cGMP  PKG

Key words:  glycyrrhizin  pulmonary arterial hypertension  right ventricular remodeling  cGMP  PKG

摘要:

目的　探讨甘草素对肺动脉高压（PAH）大鼠肺血管重塑及右心重构的影响。方法　采用腹腔注射野百合 碱（MCT）诱导法建立PAH大鼠模型。MCT建模 2 周后分别给予 60 和 20 mg/kg甘草素干预 2 周，观察大鼠右心室收 缩压（RVSP）、右心室肥厚指数（RVHI）、大鼠肺动脉组织和右心室心肌组织HE染色结果，以及检测大鼠血浆环磷酸鸟苷 （cGMP）和肺动脉组织蛋白激酶G（PKG）水平。结果 60 和 20 mg/kg甘草素均能降低MCT诱导的PAH大鼠RVSP及 RVHI，增加cGMP和PKG蛋白表达（P<0.01 或 0.05）。结论　甘草素对PAH大鼠肺血管重塑及右心重构有一定的效果。

Abstract:

Objective The aim of this study is to investigate the effect and the underlying mechanisms of glycyrrhizin on pulmonary vascular remodeling and right heart remodeling in rats with pulmonary arterial hypertension (PAH). Methods The PAH rat model was established by intraperitoneal injection of monocrotaline (MCT). After 2 weeks of MCT modeling, the rats were given 60 mg/kg and 20 mg/kg glycyrrhizin for 2 weeks. Right ventricular systolic pressure (RVSP), pulmonary vascular remodeling and right heart remodeling were observed. The plasma levels of cyclic guanosine phosphate (cGMP) and pulmonary tissue protein kinase G (PKG) were measured. Results The results showed that both 60 and 20 mg/kg glycyrrhizin decreased RVSP and RVHI in MCT-induced PAH rats, and increased the protein expression levels of cGMP and PKG (P<0.01 or 0.05). Conclusion Glycyrrhizin has certain effect on pulmonary vascular remodeling and right heart remodeling in PAH rats.