| 摘要: |
| 摘 要：目的 繁殖Scamp2-/-/ApoE-/-小鼠，并测定其血清高密度脂蛋白胆固醇（HDL-C）及肝脏三磷酸腺苷结合
盒转运体A1（ABCA1）水平。方法 将Scamp2-/-小鼠和ApoE-/-小鼠饲养于SPF级环境内，采用F0 代纯合子杂交、F1
代杂合子自交、F2 代纯合子自交方法，繁殖获得Scamp2-/-/ApoE-/-小鼠，通过PCR扩增及琼脂糖凝胶电泳鉴定基因型。
测定小鼠血清HDL-C及肝脏ABCA1 mRNA水平。结果 成功繁殖Scamp2-/-/ApoE-/-、Scamp2-/-/ApoE+/+和Scamp2+/+/
ApoE-/-小鼠。与野生型小鼠相比，ApoE-/-、Scamp2-/-、Scamp2-/-/ApoE-/-小鼠血清HDL-C及肝脏ABCA1 mRNA水平均
下降（P<0.001）；与ApoE-/-小鼠相比，Scamp2-/-/ApoE-/-小鼠血清HDL-C和肝脏ABCA1 mRNA水平分别降低了 25.26%
和 21.48%（P<0.01）。结论 成功繁殖获得Scamp2-/-/ApoE-/-小鼠；Scamp2 敲除降低了小鼠肝脏ABCA1、血清HDL-C
水平。 |
| 关键词: Scamp2 ApoE 基因敲除 高密度脂蛋白胆固醇 ABCA1 |
| DOI： |
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| 基金项目:广东省基础与应用基础研究基金自然科学基金（2020A1515010224） |
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| Breeding of Scamp2-/-/ApoE-/- mice and their detection of serum high density lipoprotein cholesterol and hepatic ABCA1 levels |
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| Abstract: |
| Abstract: Objective To breed Scamp2-/-/ApoE-/- mice and detect their serum high density lipoprotein cholesterol
(HDL-C) and hepatic ABCA levels. Methods Scamp2-/- and ApoE-/- mice were raised in SPF room, and Scamp2-/-/ApoE-/-
mice were bred using F0 homozygous hybridization and F1 heterozygous and F2 homozygous self-inbreeding. Genotypes of
offspring were identified by PCR amplification and agarose gel electrophoresis. Serum HDL-C and hepatic ABCA1 mRNA
levels were determined. Results Scamp2-/-/ApoE-/-, Scamp2-/-/ApoE+/+ and Scamp2+/+/ApoE-/- mice were successfully bred.
Serum HDL-C and hepatic ABCA1 mRNA levels were lower in Scamp2-/-, Scamp2-/- and Scamp2-/-/ApoE-/- mice than in WT
mice (P<0.001). Compared with ApoE-/- mice, serum HDL-C and hepatic ABCA1 mRNA levels were respectively decreased
by 25.26% and 21.48% in Scamp2-/-/ApoE-/- mice (P<0.01). Conclusion Scamp2-/-/ApoE-/- mice are successfully obtained.
Scamp2 knockout can reduce hepatic ABCA1 and serum HDL-C contents in mice. |
| Key words: Scamp2 ApoE gene knockout high density lipoprotein cholesterol ABCA1 |