Traditional Chinese Medicine

1 Mechanism of danshensu against the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocytes: a study based on transcriptomics and experimental validation

HUANG Shan , CHEN Xuelin , LIANG Yuyu , ZENG Qiqing , LUO Lianxiang , YANG Yajun , LIU Yanzhi , LIU Yuyu

2026, 44(4):431-441. DOI: 10.20227/j.cnki.2096-3610.2026.04.002

[Abstract](606) [HTML](2) [PDF 1.80 M](6)

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Objective To investigate the effect and mechanism of Salvianic acid A (SAA) on the proliferation and migration of human rheumatoid arthritis fibroblast-like synoviocytes (MH7A cells). Methods The effect of different concentrations of SAA on the proliferation of tumor necrosis factor-α (TNF-α)-induced MH7A cells was examined using the CCK-8 assay. The effect of SAA on the migration of TNF-α-induced MH7A cells was investigated using a scratch wound healing assay. Transcriptome sequencing analysis was employed to screen differentially expressed genes in SAAtreated TNF-α -induced MH7A cells. A protein-protein interaction (PPI) network was constructed for these genes, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to identify core genes. Molecular docking and dynamics simulations were further conducted to validate the interaction between SAA and core targets. Finally, qPCR and Western blot were used for biological validation of the core targets in the MH7A inflammatory cell model. Results TNF-α (10 μg/L) significantly induced MH7A cell proliferation (P < 0.001). Treatment with SAA at concentrations ranging from 10 to 160 µmol/L for 24, 48, and 72 hours effectively inhibited the TNF- α -induced proliferation of MH7A cells (P < 0.05). Furthermore, SAA (10 µmol/L) treatment for 24 hours significantly suppressed the TNF- α -induced migration of MH7A cells (P < 0.01). Transcriptome analysis identified 100 differentially expressed genes in SAA-treated TNF-α-induced MH7A cells. GO analysis indicated that the effects of SAA on these cells were primarily associated with focal adhesion and cadherin binding. KEGG pathway analysis suggested that the MAPK signaling pathway might be a key pathway involved in SAA's action. Molecular docking results showed a binding score of -7.290 kcal/mol between the target Superoxide dismutase 2 (SOD2) and SAA, indicating a stable and autonomous binding in silico. Molecular biological results revealed that compared to the normal group, SOD2 mRNA expression was significantly increased in TNF-α-induced MH7A cells (P < 0.0001). Compared to the model group (TNF- α alone), intervention with 10 µmol/L SAA significantly reduced SOD2 mRNA expression (P < 0.0001). Furthermore, compared with the normal group, the protein expression levels of SOD2 and CCND1 were significantly upregulated in TNF- α -induced MH7A cells (P < 0.01). Treatment with 10 µmol/L SAA significantly downregulated the protein expression of SOD2 and CCND1 (P < 0.05). Conclusion SAA can inhibit the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocytes, and its mechanism may be related to the regulation of the MAPK signaling pathway and the SOD2 gene.

2 Preparation and immunological evaluation of Ziyudisaponin-based lipid nanoparticles for enhancing antigen-specific immune responses

DENG Xiaoyu , YANG Yongqing , Li Chuxiang , XUE Jiao , PENG Xinsheng , ZHONG Xiaofang

2026, 44(4):442-449. DOI: 10.20227/j.cnki.2096-3610.2026.04.003

[Abstract](615) [HTML](0) [PDF 1.57 M](4)

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Objective To develop a Ziyudisaponin-based lipid nanovaccine (PZONs) loaded with the model antigen ovalbumin (OVA), and to evaluate its physicochemical properties, in vitro immunostimulatory activity , in vivo ability to induce specific immune responses , and its potential as an anti-tumor vaccine platform. Methods PZONs were prepared using a double emulsion-solvent evaporation method with Ziyudisaponin, OVA, lecithin and PLGA. The morphology of the nanovaccine was characterized by transmission electron microscopy (TEM), while particle size, polydispersity index (PDI), and zeta potential were determined using a Malvern Zetasizer. The stability was evaluated in phosphate-buffered saline (PBS) and serum. Bone marrow-derived dendritic cells (BMDCs) were used to assess cellular uptake, internalization mechanisms, maturation, and antigen cross-presentation via flow cytometry and confocal microscopy. Furthermore, C57BL/6 mice were immunized subcutaneously, and serum levels of OVA-specific IgG, IgG1 and IgG2a were measured by ELISA to evaluate the systemic immune response. Results PZONs were successfully prepared with uniform morphology, exhibiting spherical or sphere-like shapes, an average particle size of 140 nm, a polydispersity index of 0.24, and a zeta potential of −11 mV. The PZONs demonstrated good stability in both PBS and serum. PZONs exhibited significant cellular uptake, which was mainly mediated by clathrin-dependent endocytosis. Compared to free OVA, PZONs significantly up-regulated the expression of co-stimulatory molecules (CD40, CD80, and MHC-II) on the surface of BMDCs and enhanced antigen cross-presentation. Furthermore, PZONs elicited significantly higher titers of OVA-specific IgG, IgG1, and IgG2a, with an increased IgG2a/IgG1 ratio indicating a Th1-based immune response.Conclusion The PZONs platform successfully induce potent, Th1 biased antigen-specific immune responses, highlighting its significant potential for anti-tumor immunotherapy.

3 Research progress of Traditional Chinese Medicine in the treatment of ulcerative colitis by targeting immune cells

FENG Guoxi , ZHAO Ling

2026, 44(4):450-458. DOI: 10.20227/j.cnki.2096-3610.2026.04.004

[Abstract](446) [HTML](1) [PDF 1.30 M](5)

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Ulcerative colitis (UC) is a chronic, relapsing inflammatory bowel disease the etiology of which remains incompletely understood. Its development is closely associated with the dysregulation of mucosal immune cells. Research confirms that host-microbiota interactions regulate the differentiation and effector functions of mucosal immune cells through multiple pathways, including neuro-endocrine and metabolic mechanisms. These interactions represent key targets for understanding the underlying pathogenesis and clinical management of UC. Traditional Chinese medicine (TCM) demonstrates unique therapeutic advantages due to its holistic regulation and multi-targeted interventions. TCM has demonstrated efficacy in UC by effectively improving the gut microbiome, reversing the imbalance in mucosal immune microenvironment, restoring immune cell function, and repairing mucosal damage. This review summarizes recent advances in the therapeutic efficacy and mechanisms of TCM in regulating the immune microenvironment for UC treatment. The summarized immunomodulatory mechanisms primarily involve influencing the maturation and differentiation of macrophages or dendritic cells, inhibiting the formation of neutrophil extracellular traps, and restoring immune homeostasis of the T helper cell 17 (Th17)/regulatory T cell (Treg) and Th1/Th2.

4 Research progress on the mechanism of acupuncture in the treatment of ulcerative colitis

WANG Zirui , XU Zhirui , DUAN Yuting

2026, 44(4):459-464. DOI: 10.20227/j.cnki.2096-3610.2026.04.005

[Abstract](625) [HTML](1) [PDF 2.25 M](4)

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Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease (IBD) that affects the colon and rectum. Clinically, it is characterized by symptoms including abdominal pain, diarrhea, and bloody mucoid stools. Its pathogenesis involves the interplay of multiple factors, including genetics, environment, psychology, gut microbiota, and immune responses, and remains not fully elucidated. Acupuncture has been shown to be effective in alleviating UC symptoms, reducing disease recurrence, and improving intestinal function. Studies indicate that acupuncture can ameliorate pathological states such as spleen deficiency with excessive dampness, accumulation of damp-heat, and qi stagnation with blood stasis, thereby promoting the smooth flow of intestinal qi and harmonizing qi and blood. The underlying mechanisms may involve repairing the intestinal barrier, reducing the release of inflammatory factors including nuclear factor-kappa B and its related pathway components, and regulating gut microbiota balance.

5 Research progress on bioactive small molecules from traditional Chinese medicine and their structural optimization in the improvement of type 2 diabetes mellitus

LIU Yulan , YUAN Yanghang , WU Dudu

2026, 44(4):465-474. DOI: 10.20227/j.cnki.2096-3610.2026.04.006

[Abstract](717) [HTML](2) [PDF 1.26 M](5)

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Type 2 diabetes mellitus (T2DM) is defined by insulin resistance and progressive loss of pancreatic β‑cell function. Commonly used glucose‑lowering drugs can control blood sugar, but long‑term use often leads to cumulative side effects and diminishing efficacy. In contrast, bioactive small molecules from traditional Chinese medicine differ in several ways: they are structurally diverse, act on multiple targets, exhibit relatively low toxicity, and can work through different pathways at the same time. These properties endow them with unique value in T2DM prevention and treatment. Natural compounds such as flavonoids, alkaloids, saponins, phenolic acids and terpenoids improve insulin sensitivity through multiple routes, including modulating insulin signaling, reducing oxidative stress and inflammation, and regulating gut microbiota. However, most of these natural small molecules suffer from low oral absorption and poor targeting. To address this, three structural optimization strategies have been applied: functional group modification, (de) glycosylation, and molecular skeleton remodeling. After such modifications, these compounds exhibit better solubility, membrane permeability, and metabolic stability, and their antidiabetic effects are strengthened. This article summarizes the mechanisms, structure‑activity relationships, and recent advances in structural optimization of these TCM-derived bioactive small molecules in T2DM management, providing a reference for developing and translating next-generation antidiabetic drugs based on natural products.

6 Xiaoluowan exert anti-hepatic fibrosis effects by inhibiting the PI3K/Akt signaling pathway: based on network pharmacology and animal experiments

ZHU Chenghui , LI Junfei , JIANG Jianwei

2025, 43(5):530-539. DOI: 10.20227/j.cnki.2096-3610.2025.05.010

[Abstract](657) [HTML](35) [PDF 4.23 M](168)

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Objective To investigate the mechanism of Xiaoluowan (XLW) against liver fibrosis based on network pharmacology and animal experiments. Methods The active components of XLW and their potential targets were screened using TCMSP and HERB databases. Liver fibrosis-related targets were obtained from GeneCards, DisGeNET, and OMIM databases. The overlapping targets were analyzed via protein-protein interaction (PPI) networks on the STRING platform, followed by Gene Ontology(GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis using Metascape. Visualization was performed with Cytoscape. For animal experiments, 24 male C57BL/6 mice (6-8 weeks old) were randomly divided into control, model, and XLW groups (n = 8 per group). Liver fibrosis was induced by carbon tetrachloride (CCl4) for 8 weeks. From the 5th week onward, the XLW group received daily oral gavage of XLW decoction (5.4 mL/kg), while control and model groups received equal volume saline. After 8 weeks, mice were sacrificed to assess liver-to-body weight ratio, serum ALT/AST levels, liver pathology (histological staining), and hepatic PI3K/Akt pathway protein expression (Western blot). Results A total of 388 XLW-related targets and 3 943 liver fibrosis-related targets were identified, with 242 overlapping targets. Core targets included AKT1, TNF, TP53, and PTGS2. KEGG enrichment analysis revealed that the PI3K/Akt signaling pathway and pathways in cancer as the most significantly enriched. XLW treatment markedly reduced the liver- to-body weight ratio, improved liver function, attenuated collagen deposition, and downregulated PI3K and Akt protein expression (P < 0.05). Conclusion XLW exerts anti-fibrotic effects by modulating AKT1 core targets and inhibiting the PI3K/Akt signaling pathway.

7 Study on the mechanism of Chunpi San in the treatment of Ulcerative colitis and anal sinusitis based on network pharmacology and in vitro experiments

LI Linxuan , , LIU Qi , , LI Sikai , XING Xingxing , ZHANG Zhipeng , LIAO Zhifang , , WU Jianfang , GUI Ping , RONG Guanghong , LIAO Hongbo , ZOU Mingzhi , WU Xin ,

2025, 43(4):400-411. DOI: 10.20227/j.cnki.2096-3610.2025.04.008

[Abstract](780) [HTML](35) [PDF 1.75 M](207)

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Abstract: Objective To analyze the active ingredients and potential molecular pathways of Chunpi San (CPS) in the treatment of ulcerative colitis (UC) and anal sinusitis (AS) through network pharmacology, with experimental validation via molecular docking and in vitro functional assays. Methods Based on literature review, the active ingredients of CPS were determined. Online databases were used to obtain the active ingredients and UC/AS related targets. Venny 2.1 software was used to intersect the active ingredient targets with disease targets and upload them to the STRING analysis platform. A protein-protein interaction network (PPI) map was constructed in Cytoscape v3.10.1 software and core targets were screened. GO and KEGG enrichment analyses were performed on the intersecting targets on the WeChat online analysis platform. AutoDock Vina 1.2.3 software was used for molecular docking analysis, and Jurkat T cells were used for in vitro experimental validation. After activation with 1 μmol/L ionomycin (I) and 50 μg/L phorbol ester (P), cells were treated with 0.25 g/L and 0.50 g/L CPS extract, respectively. Cell survival rate was assessed using CCK-8 assays, the inflammatory cytokine IL-2 level in the supernatant was measured using an ELISA kit, and the relative content of MAPK pathway related proteins in cells was detected by Western blot. Results A total of 638 CPS active ingredient targets, 1 609 UC and AS related targets, 162 intersecting targets, and 24 core targets were screened. KEGG enrichment analysis showed that these mainly involve signaling pathways such as MAPK and PI3K-AKT, while GO functional analysis primarily focuses on cellular oxidative stress and signal transduction functions. The molecular docking results confirmed that the top ten active ingredients have good binding affinities with certain core targets among these. In vitro experimental results also showed that P/I can activate the release of the inflammatory cytokine IL-2 in Jurkat T cells and upregulate MAPK pathway related proteins, including p-RAF-1, p-ERK1/2, STAT3, and p-STAT3, whereas CPS extract can significantly reduce the levels of these proteins. Conclusion CPS may exert therapeutic effects on UC and AS through multiple components, targets, and pathways.

8 Total alkaloids from coptis chinensis mitigate neuronal damage induced by cerebral ischemiareperfusion in rats:involvement of the BDNF-TrkB pathway

ZHANG Xiaomin , LI Xiangyang , ZENG Xuan , PENG Haibo , WEI Nina , NIU Xianli

2025, 43(2):175-180. DOI: 10.20227/j.cnki.2096-3610.2025.02.009

[Abstract](1241) [HTML](568) [PDF 1.05 M](182)

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Objective To observe the effects of total alkaloids of coptis chinensis on neurological function impairment following cerebral ischemia-reperfusion in rats. Methods A model of middle cerebral artery occlusion (MCAO) was established in 45 healthy male SD rats, which were randomly assigned into sham operation group, model group, and treatment groups with high, medium, and low concentrations of total alkaloids of coptis chinensis, each with 9 rats. The sham operation group and model group rats were handled daily and administered saline intraperitoneally. The treatment groups were given different concentrations of total alkaloids of coptis chinensis via gavage. After 7 days of treatment, the neurological deficit scores of the rats in each group were evaluated using the 5-point scoring system proposed by longa, and the pathological changes in the hippocampal tissue were observed. The activities of superoxide dismutase (SOD), malondialdehyde (MDA), and reduced glutathione (GSH) in the hippocampal tissue were measured, and the expression of Brain-Derived Neurotrophic Factor (BDNF) and Tropomyosin Receptor Kinase B (TrkB) proteins in the hippocampal region was detected by Western blotting. Results Compared with the sham operation group, the neurologic deficit score of the model group was significantly increased (P < 0.05), the activity of SOD and the content of GSH in serum were significantly decreased (P < 0.05), while the content of MDA was significantly increased (P < 0.05). The cells of hippocampus were disordered and irregularly arranged, the reticular fibers were destroyed, the brain cells were swollen and necrotic, and formed glial scar with obvious nuclear pyknosis, and the expression of BDNF protein and TrkB protein were obviously decreased (P < 0.001). Compared with the model group, the neurologic deficit scores of rats in the total alkaloid treatment group were significantly decreased after 7 days (P < 0.05), and the glial scar in the hippocampus was significantly reduced, the activities of SOD and GSH were significantly increased (P < 0.05), and the activities of MDA were significantly decreased (P < 0.05) in each dose group of total alkaloids of coptis chinensis western blotting showed that the expression levels of BDNF and TrkB in the high and middle concentration groups were significantly higher than those in the model group (P < 0.001). Conclusion The protective effect of total alkaloids of rhizoma coptidis on hippocampal neurons can be alleviated by up-regulating the expression of BDNF and its receptor TrkB.

9 Research progress on the efficient preparation of rare ginsenosides based on enzymatic catalysis and metabolic engineering strategies

HE Tianzhu , ZOU Rui , WANG Weinan

2025, 43(1):109-118. DOI: 10.20227/j.cnki.2096-3610.2025.01.001

[Abstract](2019) [HTML](241) [PDF 1.86 M](459)

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Rare ginsenosides are the primary bioactive substances in ginseng and its processed products, possessing significant application value and market potential. However, due to their extremely low natural abundance in ginseng plants, efficient preparation strategies have become a focal point in ginseng research. By integrating modern biotransformation technologies with metabolic engineering approaches, the use of specific enzyme systems and microbial cell factories enables the efficient, targeted, and eco-friendly synthesis of rare ginsenosides. These methods not only enhance the reserves and chemical diversity of rare ginsenosides but also address environmental concerns and byproduct issues associated with traditional chemical methods. This review summarizes recent progress in enzymatic catalysis and metabolic engineering research on rare ginsenosides, providing insights and theoretical foundations to overcome industry technical barriers and promote the rapid development of ginseng-related industries.

10 Effect of paeoniflorin combined with chitosan on lipid metabolism in high-fat diet-induced non-alcoholic fatty liver disease mice

SHEN Xin , YAO Yinlian , LIU Jiahao , ZHAO Yue , JIN Hua

2025, 43(1):74-78. DOI: 10.20227/j.cnki.2096-3610.2025.01.010

[Abstract](372) [HTML](87) [PDF 1.27 M](207)

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Objective To investigate the effects of chitosan (CS) and paeoniflorin (PF) on lipid metabolism in highfat diet-induced nonalcoholic fatty liver disease (NAFLD) mice. Methods A total of 30 6-week-old male C57BL/6 mice were randomly divided into blank Control group (Ctrl group), high-fat diet group (HFD group), CS group [CS 50 mg/(kg·d)], PF group [PF 50 mg/(kg·d)]and CS +PF group [CS and PF 25 mg/(kg·d) each], with 6 mice in each group. The NAFLD model was established on a high-fat diet for 8 consecutive weeks, and then the corresponding treatment was administered by gavage. The normal diet group and the high-fat diet group were given the same volume of 0.9% normal saline. Body mass and food intake were measured every 3 days, and mice were sacrificed 8 weeks later. Body mass changes were compared between groups, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and liver fat weight were measured. Hematoxylin and eosin (HE) staining was used to observe the pathological changes in the liver and abdominal fat. Results Compared with the Ctrl group, the body weight and abdominal fat weight of the mice in the HFD group were significantly increased (P<0.000 1). CS +PF combined treatment significantly reduced both the body weight and abdominal fat weight of mice (P<0.000 1), and the effect on body weight reduction was greater than that of CS or PF alone (P<0.000 1). Additionally, the serum levels of ALT and AST in the HFD group were significantly increased compared with the Ctrl group (P<0.05). There was no significant difference in the levels of ALT and AST among the CS, PF, and CS +PF groups compared to the Ctrl group (P>0.05). There was no significant difference in liver index (liver weight/body weight) between groups (P>0.05). Conclusion The combined use of CS and PF can significantly improve lipid metabolism and reduce the accumulation of abdominal fat in NAFLD mice.

11 Protective effect of Shenqi Fuzheng liquid on high glucose-induced myocardial injury using network pharmacology and in vitro experiment

李　琴，贺旭威，张勇刚，魏文滨，陈继铭

2024, 42(4):384-389.

[Abstract](342) [HTML](7) [PDF 1.59 M](172)

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Objective To explore the potential mechanism of Shenqi Fuzheng liquid (SFL) on high glucose-induced myocardial injury (MI). Methods The active ingredients and targets of SFL, and MI genes were screened from TCMSP, GeneCards and OMIM databases, respectively. A PPI network was constructed using String database, and the intersection genes were analyzed by GO and KEGG using Metascape database. H9c2 myocardial cells were treated with 35 mmol/L glucose or adding with 1 and 5 g/L SFL. Survival rate, apoptosis and PI3K/AKT expression were detected by MTT, fluorescence staining and Western blot, respectively. Results There were 495 active ingredients of SFL, 2 148 genes of MI, and 227 intersecting genes between them. These intersecting genes were mainly involved in endocrine resistance, HIF-1, AMPK, PI3K/AKT signaling pathways. SFL increased survival rate, reduced apoptosis, and upregulated p-PI3K/p-AKT expression of injured myocardial cells. Conclusion SFL can alleviate high glucose-induced MI through PI3K/AKT signaling.

12 Salidroside protects coronary endothelial cells against ischemia-reperfusion injury by regulating mitophagy

LIANG Zheng , HUANG Yao-ying , WEN Wen , ZHONG Jian-feng , MO Shao-men , HOU Gao-xing , LI Ming-liang

2024, 42(4):375-383.

[Abstract](885) [HTML](6) [PDF 1.68 M](165)

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Objective To investigate the modulation of salidroside (Sal) on mitophagy in coronary endothelial cells (CoECs) after myocardial infarction. Methods CoECs were divided into normal control, negative control (PBS), Sal, and Sal + Chloroquine (Sal+CQ) groups; Control group was treated with normoxic condition, while the other groups with oxygen/ glucose deprivation and reperfusion (OGD/R). Cell viability, apoptosis, mitochondrial-lysosomal colocalization (MLCL), mitochondrial membrane potential (MMP), ROS, and autophagy-related protein levels were detected using CCK-8, fluorescent probe, and Western blot. Eighteen C57/BL6 mice were randomized to Sham, ischemia-reperfusion (MIRI), and MIRI+Sal groups. MIRI or MIRI+Sal group was intraperitoneally injected with normal saline or 50 mg/(kg·d) Sal 28 days before and after LAD ligation. Ejection fraction (EF), left ventricular fractional shortening (FS), myocardial fibrosis, and expression of autophagy-related proteins in infarcted coronary arteries were determined by small animal ultrasound imaging system, Masson stain and Western blot. Results Compared with PBS group, cell viability, MMP, MLCL, and expression of PINK1, Beclin1, and Parkin were increased (P<0.05), while apoptosis, ROS content, and expression of Mtfr1, P62, and LC3 II/I decreased in Sal Group (P<0.05). Compared with Sal group, cell viability, MLCL and expression of PINK1, Beclin1, and Parkin were reduced (P<0.05), while apoptosis, ROS content and expression of Mtfr1, P62, and LC3 II/I elevated (P<0.05) in Sal+CQ group. Compared with MIRI group, FS, EF and expression of PINK1, Parkin and Beclin1 were increased in infarcted coronary arteries, while myocardial fibrosis and expression of Mtfr1, P62/SQSTM1 and LC3 II/I decreased (P<0.05) in Sal+MIRI group. Conclusion Sal is protective for OGD/R-induced CoECs and infarcted coronary arteries in MIRI mice by inhibiting oxidative stress and promoting mitophagy.

13 Potential mechanism of Erzhi pill on psoriasis using network pharmacology

梁峻森

2024, 42(3).

[Abstract](661) [HTML](7) [PDF 1.72 M](251)

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Objective To analyze the active ingredients and potential molecular pathways of Erzhi pill in psoriasis using network pharmacology. Methods The active ingredients of Ligustrum lucidum and Eclipta alba in Erzhi pill were retrieved from the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform, and psoriasis-related targets were searched in DisGeNET, GeneCards and Drugbank databases. The intersections between drug and disease targets were uploaded to STRING analysis platform. PPI protein interaction network and core targets were screened by Cytoscape v3.7.2 software. The intersecting targets underwent GO and KEGG analysis using Metascape online platform. Results In L. lucidum and E. alba, there were 23 active ingredients, 211 action targets, 2 076 psoriasis targets, 118 drug-disease interaction targets and 10 core targets including TNF, AKT1, TPT1, TPP53, JUN and IL-6 genes. GO functional analysis showed they were mainly focused on nitrogen compounds, hormone and lipid responses, while mainly included PI3K-Akt, AGE-RAGE, and MAPK signaling pathways. Conclusion Erzhi pill might have therapeutic effects on psoriasis through multi-component, multi-target and multi-pathway.

14 Glycyrrhizin ameliorates right ventricular remodeling in pulmonary hypertension

ZHENG Huan , ZHENG Zhen-zhen , YANG Yi-tian , ZHANG Yuan , CHEN Ming-di , YAO Wei-min , CHENG Jun-fen , CHEN Ri-ken

2024, 42(3):251-254.

[Abstract](1260) [HTML](8) [PDF 1.26 M](310)

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Objective The aim of this study is to investigate the effect and the underlying mechanisms of glycyrrhizin on pulmonary vascular remodeling and right heart remodeling in rats with pulmonary arterial hypertension (PAH). Methods The PAH rat model was established by intraperitoneal injection of monocrotaline (MCT). After 2 weeks of MCT modeling, the rats were given 60 mg/kg and 20 mg/kg glycyrrhizin for 2 weeks. Right ventricular systolic pressure (RVSP), pulmonary vascular remodeling and right heart remodeling were observed. The plasma levels of cyclic guanosine phosphate (cGMP) and pulmonary tissue protein kinase G (PKG) were measured. Results The results showed that both 60 and 20 mg/kg glycyrrhizin decreased RVSP and RVHI in MCT-induced PAH rats, and increased the protein expression levels of cGMP and PKG (P<0.01 or 0.05). Conclusion Glycyrrhizin has certain effect on pulmonary vascular remodeling and right heart remodeling in PAH rats.

15 Comparison of the anti-viral-inflammatory of 9 kinds of traditional Chinese medicine probiotics

郭丽娜，朱佳仪，郑康帝，张　召，罗艳雯，莫丽仪，林东子

2024, 42(2):128-134.

[Abstract](587) [HTML](7) [PDF 1.16 M](274)

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Abstract: Objective To compare the anti-viral-inflammatory ability of 9 kinds of traditional Chinese medicine probiotics, and select the most effective anti-viral-inflammatory traditional Chinese medicine probiotics for the treatment of viralinflammation. Methods The cytotoxicity and biocompatibility of 9 kinds of traditional Chinese medicine probiotics were compared by using MTT methods. Poly(I:C) was used to induce macrophage RAW 264.7 to initiate an inflammatory response. The concentration of inflammatory marker interleukin-6 (IL-6) in the cell supernatant was detected to evaluate the anti-inflammatory efficacy of the traditional Chinese medicine probiotics at the cellular level. In animal experiments, 9 kinds of traditional Chinese medicine probiotics were pretreated, and then poly(I:C) was injected into zebrafish labeled with green fluorescent protein neutrophils as inducer, and the inflammation model of zebrafish swim bladder was constructed. The number of neutrophils in the swim bladder was observed and counted by fluorescence microscope. Results Among the 9 kinds of Chinese herbal probiotic compounds, Compound 8 and 9 showed dose-dependent anti-inflammatory effect on RAW264.7 cells induced by poly (I:C), while compound 4 and 7 inhibited cell inflammatory response only at high doses (P<0.01 or 0.05). The number of neutrophils in the swim bladder of zebrafish in high-dose compound 6 and 8 and medium-high-dose compound 9 were lower than those in model control group (P<0.01 or 0.05). Conclusion Among the 9 kinds of traditional Chinese medicine probiotics, No.9 had the highest efficiency for anti-inflammatory, and the highest inhibitory rate on the release of inflammatory factor IL-6 from RAW 264.7 cells induced by poly(I:C), and significantly blocked the migration of neutrophils to the swim bladder in zebrafish inflammation mode, showing a highly effective anti-inflammatory effect.

16 Clinical efficacy of Liangfu pills in common gastric diseases using Meta analysis

吴晓锋，彭素娟，温镇榕，陈　丹，冯真英

2024, 42(2):198-202，226.

[Abstract](608) [HTML](6) [PDF 1.50 M](286)

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Abstract: Objective To summarize the clinical efficacy of Liangfu pills in common gastric diseases using Meta analysis. Methods The domestic and foreign literatures on clinical efficacy of Liangfu pills in common gastric diseases were retrieved from Pubmed, EMbase, CNKI, VIP, and Wanfang databases. The Meta analysis was used to evaluate 20 eligible literatures (2 212 cases). Results The total effective rate and cure rate of Liangfu pill or its combination for gastric diseases were higher than those of western medicine or other Chinese medicine, so did for . gastric injury improvement. Conclusion Liangfu pills are superior to Western medicine in treating common gastric diseases (gastritis, peptic ulcer and gastralgia), and can promote the repair of gastric injury.

17 Possible mechanism of galangal on depression using network pharmacology and molecular docking

PENG Su-juan , FENG Zhen-ying , CHEN Dan , WANG Jian-wen , ZHOU Zhong-liu

2024, 42(1):25-33.

[Abstract](601) [HTML](5) [PDF 2.13 M](397)

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Objective To investigate the potential mechanism of galangal on depression based on network pharmacology and molecular docking. Methods The active ingredients and targets of galangal, and target genes of depression were searched from TCMSP and GeneCards databases, respectively. Protein interactions and PPI network diagram were obtained and constructed by STRING database and Cytoscape 3.9.1 software, respectively. The core targets of galangal for depression were screened, and their potential pathways were analyzed using GO function and KEGG enrichment analyses. Results Thirteen active ingredients and 169 potential targets including JUN, MAPK1, AKT1, RELA, ESR1, IL6, MAPK14, MAPK8, and RXRA were screened for depression treatment. GO analysis revealed 654 biological processes, 85 cellular components and 147 molecular functions. KEGG analysis uncovered 171 signaling pathways. Molecular docking results showed a strong binding ability between main core targets and ingredients. Conclusion Ggalangal may treat depression through multiple targets and pathways.

18 Action mechanism of Salvia miltiorrhiza in femoral head necrosis based on network pharmacology and molecular docking

CHEN Ze-yu , LIANG Hong-ying , LUO Shi-ying , ZHANG Yi-ting , CHEN yan

2024, 42(1):39-46.

[Abstract](1117) [HTML](6) [PDF 2.07 M](443)

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Abstract: Objective To explore the action mechanism of Salvia miltiorrhiza (Sm) in osteonecrosis of femoral head (ONFH) using network pharmacology and molecular docking. Methods The active components and targets of Sm were screened by TCMSP, while disease targets of ONFH were collected by OMIM, GeneCards, NCBI and DisGeNET databases. The intersection targets between Sm and ONFH were analyzed by Jevnn, GO and KEGG. The network diagram of “component-target-disease” was drawn by Cytoscape software, and the core components and intersection targets were verified by molecular docking. Results Seventy-four intersecting targets between Sm and ONFH were screened, mainly including EZH2, CYP19A1, EGFR, PTPN6 and OPRM1. There were 19 core components of Sm against ONFH, such as luteolin, salvianolic acid C, isotanshinone II, tanshinone IIA, etc. of 237 potential regulatory pathways, AGE/RAGE, fluid shear stress, atherosclerosis, and TNF were most common. Molecular docking showed that tanshinone IIA and luteolin had potential binding activities with EZH2 and EGFR. Conclusion Sm can play a role in ONFH through multiple components and targets, which may be mainly related to vascular endothelial proliferation, osteocyte differentiation and hemodynamics.

19 Visualization analysis of literature on acupuncture treatment of rheumatoid arthritis from 2013 to 2022

YAN Si-min , CAI Xiao-lin , HUANG Xin-ni , LI Hong-hui , HUANG Pei-ying , ZHANG Meng-ting , DU Yikuan , YANG Chun

2024, 42(1):47-61.

[Abstract](918) [HTML](8) [PDF 2.84 M](376)

Abstract:
Objective　To explore the research status, hot spots and frontier trends of acupuncture treatment of Rheumatoid arthritis (RA), and summarize the research direction and knowledge structure in this field. Methods　The literature on acupuncture for RA from 2013-2022 was searched in China Knowledge Network (CNKI) and Web of Science, and visualized and analyzed by using CiteSpace software and VOSviewer software as well as drawing relevant maps on authors, research institutions and keywords. Results　A total of 766 eligible CNKI papers and 178 WOS papers were included, and the number of publications generally showed an upward trend with the growth of time; the connection between the author groups of publications was not strong, and the national cooperation was centered on China, but the cooperation between countries and institutions was not close.Keyword analysis shows that the hotspot of domestic research in recent years is the mechanism of acupuncture for RA treatment, and the hotspot of foreign research in recent years is clinical observation and efficacy assessment. Conclusion　The treatment of RA by acupuncture is multi-target and multi-pathway, and in future research, new research perspectives such as metabolomics, mechanism research, key pathways and related metabolic networks in the treatment of RA by acupuncture should be actively explored to provide more reference basis for clinical treatment of RA.

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Traditional Chinese Medicine

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