羊栖菜多糖脱色工艺优化及其抗氧化活性评价

Optimization decolorization process of polysaccharide from Sargassum fusiforme and its antioxidant activity

摘要:

目的 探究羊栖菜多糖的脱色工艺及其抗氧化活性。方法 采用活性炭、过氧化氢、D101型大孔树 脂和AB-8型大孔树脂4种脱色方法对羊栖菜多糖进行脱色。在单因素试验的基础上，以多糖保留率和脱蛋白率为 指标，通过响应面法优化羊栖菜多糖的脱色工艺，评价脱色前后羊栖菜多糖的体外抗氧化活性。结果 对比4种脱 色方法，选择脱色效果较优的 AB-8型大孔树脂脱色法，通过响应面试验设计得出 AB-8型大孔树脂脱色法的最佳 脱色工艺条件为：树脂添加量为10%，多糖溶液pH为7，脱色时间为2 h，脱色温度为30 ℃。在此条件下，多糖保留 率为 72.60%，脱蛋白率为 69.27%。体外抗氧化活性结果显示，脱色后的羊栖菜多糖对 1，1-二苯基-2-三硝基苯肼 （DPPH）自由基和羟基自由基的最大清除率分别达到 71.32% 和 78.95%。羊栖菜多糖对 DPPH 自由基和羟基自由 基的清除能力弱于抗坏血酸（P<0.05））结论 AB-8型大孔树脂脱色法对羊栖菜多糖的脱色效果优于其他3种脱色 方法，脱色前后羊栖菜多糖均具有一定的抗氧化活性。

Abstract:

Objective To study the decolorization process of Sargassum fusiforme polysaccharide and its antioxidant activity. Methods Four decolorization methods, including activated carbon, hydrogen peroxide, D101 macroporous resin and AB-8 macroporous resin, were employed to decolorize the Sargassum fusiforme polysaccharide. On the basis of single-factor experiments, the decolorization rate and polysaccharide retention rate were used as the indexes to optimize the decolorization process of Sargassum fusiforme polysaccharide using response surface methodology. Furthermore, the in vitro antioxidant activities of Sargassum fusiforme polysaccharide were evaluated both before and after decolorization. Results Upon comparing the four decolorization methods, the AB-8 macroporous resin method, which demonstrated the best decolorization effect, was selected. The optimal decolorization process conditions for this method were obtained through the response surface test design as follows: the resin addition was 10%, the polysaccharide solution pH was 7, the decolorization time was 2 h, and the decolorization temperature was 30 ℃ . Under these conditions, the polysaccharide retention rate was 72.60%, and the deproteinization rate was 69.27%. The results of the in vitro antioxidant activity demonstrated that the maximum scavenging rates of DPPH radicals and hydroxyl radicals from the Sargassum fusiforme polysaccharide after decolorization could reach 71.32% and 78.95%, respectively. The scavenging ability of Fargesia fasciata polysaccharides on DPPH free radicals and hydroxyl free radicals was weaker than that of ascorbic acid (P<0.05). Conclusion The decoloration effect of the AB-8 macroporous resin decolorization method on Sargassum fusiforme polysaccharide was superior to that of the other three decolorization methods, and the Sargassum fusiforme polysaccharides before and after decolorization exhibited moderate antioxidant activities, which can be developed and utilized as natural antioxidants.