芍药苷联合壳聚糖对高脂饲料诱导的非酒精性脂肪肝小鼠脂质代谢的影响

Effect of paeoniflorin combined with chitosan on lipid metabolism in high-fat diet-induced non-alcoholic fatty liver disease mice

摘要:

目的 探讨壳聚糖（CS）和芍药苷（PF）及其联合应用对高脂饮食诱导的非酒精性脂肪肝病（NAFLD） 小鼠脂质代谢的影响。方法 选取 6 周龄雄性 C57BL/6 小鼠 30 只，随机分为空白对照组（Ctrl 组）、高脂饮食组 （HFD 组）、CS 组［CS 50 mg/（kg·d）］、PF 组［PF 50 mg/（kg·d）］和 CS+PF 组［CS 和 PF 各 25 mg/（kg·d）］，每组 6 只。 连续8周高脂饮食建立NAFLD模型，随后通过灌胃给予相应处理，Ctrl组和HFD组给予等体积0.9%生理盐水。每 3 天测量 1 次身体质量，8 周后处死小鼠，比较各组的身体质量变化，并检测血清谷丙转氨酶（ALT）和谷草转氨酶 （AST）水平及肝脏脂肪质量。采用 HE染色观察肝脏和腹部脂肪的病理变化。结果 与 Ctrl组相比，HFD组小鼠 身体质量、腹部脂肪质量均显著增加（P<0.000 1）。CS+PF联合治疗显著减轻了小鼠的身体质量和腹部脂肪质量 （P<0.000 1），且减轻小鼠身体质量的效果优于单独使用 CS或 PF（P<0.000 1）；与 Ctrl组相比，HFD 组小鼠的血清 ALT 和 AST 水平显著上升（P<0.05）。CS、PF 及 CS+PF 组的 ALT 和 AST 水平与 Ctrl 组相比差异无统计学意义 （P>0.05）。各组间肝脏指数（肝脏质量/身体质量）比较差异无统计学意义（P>0.05）。结论 CS和PF联合使用能 显著改善NAFLD小鼠的脂质代谢。

Abstract:

Objective To investigate the effects of chitosan (CS) and paeoniflorin (PF) on lipid metabolism in highfat diet-induced nonalcoholic fatty liver disease (NAFLD) mice. Methods A total of 30 6-week-old male C57BL/6 mice were randomly divided into blank Control group (Ctrl group), high-fat diet group (HFD group), CS group [CS 50 mg/(kg·d)], PF group [PF 50 mg/(kg·d)]and CS +PF group [CS and PF 25 mg/(kg·d) each], with 6 mice in each group. The NAFLD model was established on a high-fat diet for 8 consecutive weeks, and then the corresponding treatment was administered by gavage. The normal diet group and the high-fat diet group were given the same volume of 0.9% normal saline. Body mass and food intake were measured every 3 days, and mice were sacrificed 8 weeks later. Body mass changes were compared between groups, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and liver fat weight were measured. Hematoxylin and eosin (HE) staining was used to observe the pathological changes in the liver and abdominal fat. Results Compared with the Ctrl group, the body weight and abdominal fat weight of the mice in the HFD group were significantly increased (P<0.000 1). CS +PF combined treatment significantly reduced both the body weight and abdominal fat weight of mice (P<0.000 1), and the effect on body weight reduction was greater than that of CS or PF alone (P<0.000 1). Additionally, the serum levels of ALT and AST in the HFD group were significantly increased compared with the Ctrl group (P<0.05). There was no significant difference in the levels of ALT and AST among the CS, PF, and CS +PF groups compared to the Ctrl group (P>0.05). There was no significant difference in liver index (liver weight/body weight) between groups (P>0.05). Conclusion The combined use of CS and PF can significantly improve lipid metabolism and reduce the accumulation of abdominal fat in NAFLD mice.