奥沙利铂联合X射线照射建立小鼠再生障碍性贫血模型

Establishment of a mouse model of aplastic anemia using oxaliplatin and X-ray irradiation

摘要:

目的 应用奥沙利铂联合X射线照射建立小鼠再生障碍性贫血模型。方法 将 10 只小鼠随机分为对照组 和模型组，每组 5 只。模型组于第 1、3、5、7、9、11、13、15、17、19 天腹腔注射 3 mg/kg奥沙利铂，第 7、14 天给予 3.0 Gy X射线照射；对照组于相同时间点腹腔注射等剂量生理盐水，经铅砖遮挡进行假照射。观察小鼠一般状态及身体质量， 第 20 天进行血液检查、脾脏指数及脾、骨髓病理检查。结果 与对照组相比，模型组小鼠在第 1 次X射线照射后，出现精 神萎靡；第 2 次X射线照射后，毛色无光泽，耳、四肢逐渐苍白，身体质量增长速度明显下降；脾体积减小、脾脏指数降低 （P<0.001）；外周血白细胞、红细胞、血红蛋白、血小板水平显著下降（P<0.001 或 0.01）；HE染色显示脾组织结构疏松， 骨髓有核细胞减少，存在大量脂肪空泡。结论 奥沙利铂联合X射线可建立再生障碍性贫血小鼠模型。

Abstract:

Objective To establish a mouse model of aplastic anemia using oxaliplatin and X-ray irradiation. Methods Ten mice were randomly divided into control group (n=5) and model group (n=5). The model group were intraperitoneally injected with 3 mg/kg oxaliplatin at 1, 3, 5, 7, 9, 11, 13, 15, 17, and 19 days, and radiated with 3.0 Gy X-ray at 7 and 14 days; while the control group received intraperitoneal injection of the same dose of normal saline and sham irradiation with lead brick shielding at the same timepoints. The general condition and body weight of mice were observed. On the 20th day, blood examination, spleen index and pathological examination of spleen and bone marrow were performed. Results Compared with control group, model group showed listlessness after the first X-ray irradiation; dull fur color, gradual paleness of ears and limbs, and decreased weight growth rate after the second X-ray irradiation. The spleen volume and index decreased (P<0.001). White blood cells, red blood cells, hemoglobins, and platelets of peripheral blood reduced dramatically (P<0.001 or 0.01). Hematoxylin-eosin (HE) stain revealed loose structure of spleen and decreased nucleated cells with numerous fat vacuoles of bone marrow. Conclusion Combined oxaliplatin and X-ray irradiation can create an aplastic anemia model in mice.