NEIL3 调控AKT/GSK3β/Cyclin D1 信号通路诱导肺腺癌细胞增殖

NEIL3 induces the proliferation of lung adenocarcinoma cells via AKT/GSK3β/Cyclin D1 signaling pathway

摘要:

目的 探讨NEIL3（Nei核酸内切酶VIII样蛋白 3）在肺腺癌组织中的表达，明确其对肺腺癌细胞增殖的影 响和分子机制。方法 免疫组化检测肺腺癌组织NEIL3 的表达，分析NEIL3 表达与患者临床病理参数和生存预后的关 系。采用short hairpin RNA（shRNA）慢病毒载体转染技术干扰肺腺癌细胞A549 中NEIL3 的表达后，平板克隆实验、5- 乙炔基-2 ′ 脱氧尿嘧啶核苷（EdU）实验和流式周期实验检测细胞增殖的改变；Western blot检测增殖相关分子Cyclin D1 和信号通路 AKT/GSK3β/β-catenin 的改变。 结果 NEIL3 表达与肺腺癌患者的临床分期、T 分期及生存时间有关 （P<0.05）。干扰NEIL3 表达诱导A549 细胞出现G0/G1 期阻滞，抑制细胞增殖（P<0.05）。机制上，抑制NEIL3 可促进 Cyclin D1 蛋白酶体降解从而下调其蛋白水平；干扰NEIL3 可抑制AKT/GSK3β/β-catenin信号通路的激活（P<0.05）。结 论 NEIL3 诱导肺腺癌细胞的增殖，机制可能与其调控AKT/GSK3β信号通路抑制Cyclin D1 蛋白酶体降解有关。

Abstract:

Abstract: Objective The aim of this study is to investigate the expression and clinical significance of NEIL3(Nei like DNA glycosylase 3) in lung adenocarcinoma tissue, and to identify the effect of NEIL3 on the proliferation of lung adenocarcinoma cells and the underlying molecular mechanism. Methods Immunohistochemistry was used to detect the expression of NEIL3 in lung adenocarcinoma tissues, and the relationship between NEIL3 expression and the clinicopathological parameters, as well as the prognosis of patients were analyzed. After knockdown of NEIL3 expression in lung adenocarcinoma cell A549 through short hairpin RNA (shRNA), colony formation assay, 5-ethynyl-2′-deoxyuridine (EdU) assay and flow cytometry were employed to detect the alterations in cell proliferation. Changes of proliferation-related molecules Cyclin D1 and AKT/GSK3β/β-catenin signaling pathway were detected by using Western blot. Results NEIL3 expression was correlated with clinical stage, T stage and the overall survival time of lung adenocarcinoma patients (P<0.05). Down-regulation of NEIL3 induced G0/G1 phase arrest in A549 cells and inhibited cell proliferation in vitro (P<0.05). Mechanically, inhibition of NEIL3 promoted the proteasomal degradation of Cyclin D1. Additionally, down-regulation of NEIL3 suppressed the activation of the AKT/GSK3β/β-catenin signaling pathway(P<0.05). Conclusion NEIL3 may promote the proliferation of lung adenocarcinoma cells through the activation of AKT/GSK3β signaling pathway, which in turn inhibits the proteasomal degradation of Cyclin D1.