网络药理学分析二至丸治疗银屑病的潜在机制

Potential mechanism of Erzhi pill on psoriasis using network pharmacology

摘要:

目的 使用网络药理学分析二至丸治疗银屑病的有效成分和潜在分子通路。方法 利用中药系统药理学数据库与分析平台（TCMSP）检索二至丸中女贞子和墨旱莲的有效成分，在DisGeNET、GeneCards、Drugbank数据库中检索得到银屑病相关靶点，将药物靶点与疾病靶点取交集后上传至STRING分析平台，在Cytoscape v3.7.2软件绘制PPI蛋白互作网络并筛选核心作用靶点，在Metascape在线分析平台对交集靶点进行GO功能富集和KEGG通路富集分析。结果筛选到女贞子和墨旱莲有效成分23个，作用靶点211个，银屑病靶点2 076个，药物与疾病共同作用靶点118个，核心作用靶点10个，其中包括TNF、AKT1、TP53、JUN、IL-6等基因。GO功能分析主要集中在氮化合物反应、激素反应、脂质反应等，KEGG富集分析显示信号通路主要包括PI3K-Akt、AGE-RAGE、MAPK通路等。结论 二至丸可能通过多成分、多靶点和多途径对银屑病发挥治疗作用。

Abstract:

Objective To analyze the active ingredients and potential molecular pathways of Erzhi pill in psoriasis using network pharmacology. Methods The active ingredients of Ligustrum lucidum and Eclipta alba in Erzhi pill were retrieved from the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform, and psoriasis-related targets were searched in DisGeNET, GeneCards and Drugbank databases. The intersections between drug and disease targets were uploaded to STRING analysis platform. PPI protein interaction network and core targets were screened by Cytoscape v3.7.2 software. The intersecting targets underwent GO and KEGG analysis using Metascape online platform. Results In L. lucidum and E. alba, there were 23 active ingredients, 211 action targets, 2 076 psoriasis targets, 118 drug-disease interaction targets and 10 core targets including TNF, AKT1, TPT1, TPP53, JUN and IL-6 genes. GO functional analysis showed they were mainly focused on nitrogen compounds, hormone and lipid responses, while mainly included PI3K-Akt, AGE-RAGE, and MAPK signaling pathways. Conclusion Erzhi pill might have therapeutic effects on psoriasis through multi-component, multi-target and multi-pathway.