基于海藻酸钠复合苹果多酚的化学光热抗菌敷料的制备及抗菌效果观察

Preparation and antibacterial efficacy of chemical and photothermal antibacterial dressing based on sodium alginate composite apple polyphenols

摘要:

目的 制备一种新型的化学/光热联合治疗细菌感染的非抗生素伤口抗菌敷料。方法 以Fe3+、海藻酸钠 （SA）和苹果多酚（APP）3 种天然物质为原料，通过冷冻干燥原位非共价交联构建海绵状骨架制备一种海藻酸钠复合苹 果多酚的光热抗菌敷料SA/APP/Fe3+（SAF）。以体内外试验验证其生物安全性、止血能力及杀菌能力。结果 在近红外 辐射下，SAF抗菌敷料能杀死近 80%金黄色葡萄球菌和 85%大肠杆菌。SAF抗菌敷料的细胞存活率高于 95%，溶血率 为 6.75%；与纱布相比，SAF抗菌敷料的失血量减少了 26%，止血时间减少了 76%。结论 制备的抗菌敷料具有较强的 抗菌效果，且生物安全性高、止血能力强。

Abstract:

Objective To prepare a new type of chemical and photothermal non-antibiotic wound antibacterial dressing for bacterial infections. Methods The sponge-ike skeleton of photothermal antibacterial dressing SA/APP/Fe3+ (SAF) was prepared by freeze-drying in situ and non-covalent crosslinking using Fe3+, sodium alginate, and apple polyphenol. Its biological safety, hemostatic ability and bactericidal ability were testified by in vitro experiments. Results Under near-infrared radiation, SAF antibacterial dressing killed nearly 80% of Staphylococcus aureus and 85% of Escherichia coli. The cell survival rate of SAF antibacterial dressing was more than 95%, and hemolysis rate was 6.75%. Compared with gauze, SAF antibacterial dressing reduced blood loss by 26% and hemostatic time by 76%. Conclusion This antibacterial dressing has strong antibacterial effect, high biological safety and hemostatic ability.