Abstract:Objective To investigate the impact of different feeds on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice. Methods Sixty-eight mice were randomly allocated into two groups, each receiving feeds A (n=42) and B (n=26). After adaptive feeding, they were randomly divided into control group [ACtr group (n=21) and BCtr group (n=6)] and DSS group [A25 DSS group (n=21), B25 DSS group (n=6), B30 DSS group (n=6), B35 DSS group (n=8)]. Control group drank sterile water freely for 11 days. DSS group drank the corresponding concentration of DSS solution freely for 7 days, and then sterile water for 4 days. At the end of the experiment, the body mass, disease activity index (DAI), colitis symptoms and mortality were evaluated. Results After modeling, there was no significant difference in body weight among A25 DSS, B25 DSS, B30 DSS and B35 DSS groups (P>0.05). DAI of A25 DSS group was significantly higher than that of B25 DSS group (P<0.0001), but similar to B30 DSS and B35 DSS groups (P>0.05). The mortality was significantly higher in A25 DSS group than in B25 DSS, B30 DSS and B35 DSS groups (P<0.001). Conclusion High nutrient diet may be beneficial to the establishment of DSS-induced UC model.
