血清oxLDL、CTRP5、MCP-1 表达水平与高血压患者颈动脉粥样硬化的相关性分析

Correlation analysis of serum oxLDL, CTRP5, MCP-1 expression levels and carotid atherosclerosis in hypertensive patients

关键词:  高血压  颈动脉粥样硬化  氧化低密度脂蛋白  补体C1q/肿瘤坏死因子相关蛋白 5  单核细胞趋化蛋白-1  诊断价值

Key words:  hypertension  carotid atherosclerosis  oxidized low-density lipoprotein  complement C1q/tumor necrosis

摘要:

目的　探究血清氧化低密度脂蛋白（oxLDL）、补体C1q/肿瘤坏死因子相关蛋白 5（CTRP5）、单核细胞趋化 蛋白-1（MCP-1）表达水平与高血压颈动脉粥样硬化（CAS）的相关性。方法　 选取 143 例高血压患者为研究对象，根 据颈动脉内膜中层厚度（CIMT）将其分为正常组（n=61）和CAS组（n=82），另选同期体检的 87 例健康者作为对照组。 ELISA法测定血清oxLDL、CTRP5、MCP-1 水平；血清oxLDL、CTRP5、MCP-1 水平与CIMT的关系用Pearson相关性 分析法；logistic回归法探究高血压患者发生CAS的影响因素；血清oxLDL、CTRP5、MCP-1 对高血压CAS的诊断价值 使用受试者工作特征（ROC）曲线进行探究。结果　 对照组、正常组、CAS组血清oxLDL、CTRP5、MCP-1 水平依次升 高（P<0.05）。Pearson结果显示血清oxLDL、CTRP5、MCP-1 与CIMT呈显著正相关（r=0.526、0.592、0.646，P<0.05）。 logistic回归分析结果显示：同型半胱氨酸（Hcy）、高密度脂蛋白（HDL-C）、oxLDL、CTRP5、MCP-1 均为高血压患者发生 CAS的影响因素（P<0.05）。oxLDL、CTRP5 与MCP-1 联合诊断的AUC均显著大于oxLDL、CTRP5 和MCP-1 的单独诊 断（P<0.05）。结论　血清oxLDL、CTRP5、MCP-1 水平在高血压CAS患者中均升高，3 者表达水平与CIMT呈正相关， 且 3 者在诊断高血压CAS具有较高诊断效能。

Abstract:

Objective The aim of this study is to determine the correlation between serum oxidized low-density lipoprotein (oxLDL), complement C1q/tumor necrosis factor related protein 5 (CTRP5) and monocyte chemotactic protein-1 (MCP-1) and hypertensive carotid atherosclerosis (CAS). Methods Based on the carotid intima-media thickness (CIMT), 143 hypertensive patients were divided into two groups: a normal group (n=61) and a CAS group (n=82). Additionally, 87 healthy individuals who underwent physical examinations were included in the control group. ELISA method was applied to measure serum levels of oxLDL, CTRP5, and MCP-1. The relationship between serum oxLDL, CTRP5, MCP-1 levels and CIMT was analyzed using Pearson correlation method. Logistic regression method was applied to explore the influencing factors of CAS occurrence in hypertensive patients. The diagnostic value of serum oxLDL, CTRP5, and MCP-1 in hypertensive CAS was investigated using receiver operating characteristic (ROC) curves. Results The levels of serum oxLDL, CTRP5, and MCP-1 in the control group, normal group, and CAS group all increased sequentially (P< 0.05). Pearson analysis showed a significant positive correlation between serum oxLDL, CTRP5, MCP-1 and CIMT (r=0.526, 0.592, 0.646, P<0.05). Logistic regression analysis suggested that homocysteine (Hcy), high-density lipoprotein (HDL-C), oxLDL, CTRP5, and MCP-1 were all influencing factors for the occurrence of CAS in hypertensive patients (P<0.05).The AUC of oxLDL, CTRP5 and MCP1 combined diagnosis was significantly higher than that of each index alone diagnosis (P<0.05). Conclusion The levels of serum oxLDL, CTRP5, and MCP-1 are all elevated in hypertensive CAS patients, and their expression levels are positively correlated with CIMT. Moreover, these three factors demostrate high diagnostic efficacy in diagnosing hypertensive CAS.